Arquivo Brasileiro de Medicina Veterinária e Zootecnia
versión On-line ISSN 1678-4162
HORTA, M.C. et al. Rickettsia felis (Rickettsiales: Rickettsiaceae) in Ctenocephalides felis felis (Siphonaptera: Pulicidae) in the State of São Paulo, Brazil. Arq. Bras. Med. Vet. Zootec. [online]. 2005, vol.57, n.3, pp. 321-325. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352005000300008.
Samples of 10 and 14 Ctenocephalides felis felis fleas were collected on dogs from Pedreira and Mogi das Cruzes municipalities, respectively, in the State of São Paulo, Brazil, for detection of Rickettsia spp. Individual fleas were submitted to Polymerase Chain Reaction targeting the 17-kDa and the 190-kDa (OmpA) genes of Rickettsiae. This later gene is specific for spotted fever group. Nine fleas from Pedreira (90%) and four fleas from Mogi das Cruzes (28%) were positive for the 17-kDa gene, and eight fleas from Pedreira (80%) and four from Mogi das Cruzes (28%) were positive for 190-kDa gene. The nucleotide sequence of the 190-kDa products of one flea from Pedreira and one flea from Mogi das Cruzes were 100% identical to each other, and when compared to the GenBank Data, they were 100% identical to the 190-kDa sequence of R. felis. This was the first report of its occurrence in the State of São Paulo.
Palabras clave : Rickettsia felis; Ctenocephalides felis felis; dog; 190-kDa gene; Brazil.