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Arquivo Brasileiro de Medicina Veterinária e Zootecnia
On-line version ISSN 1678-4162
NEVES, M.M. et al. Preservation of chinchilla genital system (Chinchilla lanigera) for microscopic evaluation. Arq. Bras. Med. Vet. Zootec. [online]. 2006, vol.58, n.6, pp. 1247-1248. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352006000600042.
The preservation of chinchilla genital organs using a fixed solution of paraformaldehyde 10% buffered and a saturated solution of Bouin during 4, 12 and 18 hours of fixation, inclusion in paraffin and staining with hematoxylin-eosin was evaluated. The Bouin solution with 12 hours of fixation was the best protocol of fixation for the ovaries, oviduct, uterus and vagina, resulting in little tissue retraction and better cellular integration when compared to the other fixing times (4 and 18 hours). The fixation with paraformaldehyde showed good results of tissues fixation. Chinchilla genital organs can be preserved with paraformaldehyde 10% buffered and Bouin solution using 12 hours of fixation.
Keywords : chinchilla; Chinchilla lanigera; female genital system; fixing solution; light microscopy.