Lactating Holstein cows (n=158), at 213±112 days in milking and averaging 26±9kg of milk per day, were randomly assigned to one of three treatment groups: control (CG, n=52, saline), GnRH (GG, n=55, 100g gonadorelin), and hCG (HG, n=51, 2500IU) given five days after artificial insemination (AI). Rectal temperature was taken at the moment of AI and blood samples were collected five, seven, and 12 days after AI. Pregnancy was determined between 42 and 49 days after AI. Concentration of progesterone (P4) in serum (ng/ml, mean±SE) for CG, GG, and HG were, respectively, 2.7±0.4, 2.5±0.4, and 3.2±0.5 on day 5; 4.8±0.4, 4.2±0.4, and 5.7±0.5 on day 7; and 5.2±0.4, 6.9±0.4, and 8.5±0.5 on day 12 after AI. P4 concentration had proportional increase in serum between days 5 and 7 after AI (CG: 178%, GG: 168%, and HG: 178%), suggesting that the treatments did not induce a luteotropic effect on the existing corpus luteum (CL). Concentrations of P4 increased between days 7 and 12 in cows treated with GnRH and hCG (GG: 164%, and HG: 149%, P<0.01); but not in control cows (GC: 18%, P=0.31), suggesting that a new CL was formed. Treatments with GnRH or hCG increased conception rates in cows with rectal temperature below 39.7°C (CG: 10.1%, n=26, GG: 36.8%, n=27; and HG: 32.8%, n=21), but not in cows with rectal temperature above 39.7°C (CG: 15.2%, n=26; GG: 17.8%, n=28; and HG: 24.4%, n=30). These data suggest that high body temperature masked the positive impact of treatment with GnRH or hCG on day 5 after AI on conception.
dairy cow; conception; progesterone; hCG; GnRH
