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Arquivo Brasileiro de Medicina Veterinária e Zootecnia
On-line version ISSN 1678-4162
SALVARANI, F.M. et al. In vitro evaluation of Clostridium septicum alpha toxoidAvaliação in vitro de toxoide alfa de Clostridium septicum. Arq. Bras. Med. Vet. Zootec. [online]. 2010, vol.62, n.4, pp.778-783. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352010000400002.
Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration.
Keywords : seroneutralization; mouse; guinea pig; cell culture; vaccine.