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Arquivo Brasileiro de Medicina Veterinária e Zootecnia
On-line version ISSN 1678-4162
TONIOLLI, R.; TONIOLLO, G.H.; FRANCESCHINI, P.H. and MORATO, F.M.A.C.. Use of powder coconut water as extender (ACP-103®) for boar semen longer preservation: in vitro and in vivo evaluations. Arq. Bras. Med. Vet. Zootec. [online]. 2010, vol.62, n.5, pp.1072-1079. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352010000500008.
The semen of eight boars was collected and a total of 1.75x109 spermatozoa were separated from each ejaculate, obtaining a concentration of 35x106sptz/mL. The Beltsville Thawing Solution (BTS) was used as control, being tested the powder coconut water as extender (PCW). The day of collection was considered day zero (D0), and the semen was conserved for five days, with analyses on D0 and on four subsequent days (D1, D2, D3, and D4). The evaluation of spermatic quality was based on the results of spermatozoa vigour, cells motility, spermatic morphology, plasmatic membrane integrity, and fertility. The evaluations of the in natura semen presented mean values of spermatozoa vigour (4.1) and mobile spermatozoids percentage (91%) above the demanded minimum parameters (3.0 and 70%) for its use in artificial insemination programs without influencing on the analyzed variables. The mean results of the osmotic resistance were excellent, with 71.3% of spermatozoids with coiled tail. There were no differences between the two extenders tested for the characteristic cells with intact acrossome (BTS = 67.1%; PCW = 71.2%). The semen diluted in PCW extender presented a higher number of alive cells (77.7%) with a complete plasmatic membrane (74.2%) after conservation period. The choice of PCW extender is advisable for the routine use in laboratories that work with conservation of swine semen. In spite of the good results in vitro with the PCW extender, the BTS presented the best fertility results, 86.7% and 96.7%, respectively.
Keywords : swine; semen; cocconut water; conservation; morphology; fertility.