SciELO - Scientific Electronic Library Online

vol.63 issue4The effect of furosemide and phenylbutazone association on the fluid and electrolyte characteristics of horses before and after a raceNeurological disorder in cattle associated with bovine herpesvirus 4 author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand



Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google


Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Print version ISSN 0102-0935


FREITAS, P.P.S. et al. Rotavirus in cattle: risk factors, prevalence and antigenic characterization from dairy calves' samples in São Paulo State, Brazil. Arq. Bras. Med. Vet. Zootec. [online]. 2011, vol.63, n.4, pp. 820-827. ISSN 0102-0935.

The study on rotavirus infection prevalence in calves was undertaken in 51 dairy cattle herds, randomly selected, in a dairy area in the state of São Paulo. One hundred and three samples of feces from calves with diarrhea and 308 samples of feces from calves free from the disease, age ranging from 1 to 45 days, were collected from May 31st to October 20th 2003. Stool samples were analyzed through immunoenzymatic assay techniques (IEA) and polyacrylamide gel electrophoresis (PAGE). Rotavirus prevalence rate of 21.6% (11/51) was detected by IEA in cattle herds and 6.7% (27/404) in calf population. Rotavirus infection was diagnosed in calves with diarrhea (18.4%; 19/103) and in clinically healthy calves (2.7%; 8/301). The highest infection frequency was found in calves aged 1 to 15 days. There is an inverse relationship between positive frequency and age of animals (P<0.05). Factors which may affect rotavirus prevalence in herds, such as type of meals (manual milk supply or calf with dame), enclosure (individual or collective pens), herd size (number of matrixes) and age have been analyzed by the chi-square test, and significantly affected infection frequency (p<0.05). RNA from 27 positive samples by PAGE were classified in seven electrophorotypes and showed the rotavirus' extensive genomic diversity. Rotavirus positive samples genotyping was undertaken through the reverse transcription-polymerase chain reaction (RT-PCR), underpinning infections by special genotypes G6P[5] and G10P[11].

Keywords : calf; rotavirus; diarrhea; risk factors; genotyping.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License