Arquivo Brasileiro de Medicina Veterinária e Zootecnia
On-line version ISSN 1678-4162
CARVALHO, A.F. et al. Molecular and phenotypic characterization of shiga toxin producing Escherichia coli (STEC) non-O157 strains from bovine feces and carcass. Arq. Bras. Med. Vet. Zootec. [online]. 2012, vol.64, n.4, pp.881-886. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352012000400014.
In the present study 100 rectal and 100 carcass swabs were collected from bovines at slaughterhouses in São Paulo state, and the total of 326 E. coli strains were identified (163 from rectal samples and 163 from carcass samples). All the isolates were submitted to PCR for Stx1 and Stx2 toxin gene detection and all strains were identified as non-O157 and phenotyped by the citotoxicity test in Vero cells. Out of 26 strains that presented only the stx1 gene, 56 that presented only the stx2 gene and 30 that presented both genes, 17 (65.4%), 42 (75%) and 22 (73.3%), respectively, were positive for the citotoxicity test. There was no statistically significant difference among these three toxinotyping profiles and positivity in the citotoxicity test, but the results show high frequency of virulence factor expression of bovine.
Keywords : bovine; Escherichia coli; STEC; feces; carcass.