SciELO - Scientific Electronic Library Online

vol.64 issue4Evaluation of the integrated parasite control in a semi-intensive goat farming in the region of Santa CatarinaSupplementation of Nelore steers under grazing during the rainy/dry transition period author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Arquivo Brasileiro de Medicina Veterinária e Zootecnia

On-line version ISSN 1678-4162


ALVES, L.A.O. et al. Production of antigen and p28 protein separation by microfiltration serial for serodiagnosis of caprine arthritis encephalitis by enzyme immunoassay. Arq. Bras. Med. Vet. Zootec. [online]. 2012, vol.64, n.4, pp.935-942. ISSN 1678-4162.

This study aimed to produce an antigen (Ag) from the culture of goat synovial membrane cells (MSC) infected by CAEV through serial microfiltering technique replacing ultra ultracentrifugation in sacarosis Mattress (UCCS) for the indirect diagnosis ELISA tests (i ELISA). Samples of 188 sera from goats previously examined by Western Blot (WB) with Ag UCCS were submitted to analysis by i ELISA with new antigen produced, demonstrating an accordance of 92% in relation to UCCS antigen. The specificity and sensitivity relating to WB were of 95,65% and 88, 5% respectively. The new technique created from the microfiltering is effective and with low cost for the serological antibodies diagnosis of CAEV comparing to the ultracentrifuged one, presenting, therefore, as a viable alternative for purified antigen of lentivirus in small ruminants.

Keywords : caprine; CAEV; ELISA; lentivirus; Western blot.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License