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Arquivo Brasileiro de Medicina Veterinária e Zootecnia
On-line version ISSN 1678-4162
ALVES, L.A.O. et al. Production of antigen and p28 protein separation by microfiltration serial for serodiagnosis of caprine arthritis encephalitis by enzyme immunoassay. Arq. Bras. Med. Vet. Zootec. [online]. 2012, vol.64, n.4, pp.935-942. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352012000400021.
This study aimed to produce an antigen (Ag) from the culture of goat synovial membrane cells (MSC) infected by CAEV through serial microfiltering technique replacing ultra ultracentrifugation in sacarosis Mattress (UCCS) for the indirect diagnosis ELISA tests (i ELISA). Samples of 188 sera from goats previously examined by Western Blot (WB) with Ag UCCS were submitted to analysis by i ELISA with new antigen produced, demonstrating an accordance of 92% in relation to UCCS antigen. The specificity and sensitivity relating to WB were of 95,65% and 88, 5% respectively. The new technique created from the microfiltering is effective and with low cost for the serological antibodies diagnosis of CAEV comparing to the ultracentrifuged one, presenting, therefore, as a viable alternative for purified antigen of lentivirus in small ruminants.
Keywords : caprine; CAEV; ELISA; lentivirus; Western blot.