Arquivo Brasileiro de Medicina Veterinária e Zootecnia
On-line version ISSN 1678-4162
VOLPATO, R. et al. Immunohistochemical of the cervix and uterus of bitches diagnosed with pyometra. Arq. Bras. Med. Vet. Zootec. [online]. 2012, vol.64, n.5, pp.1109-1117. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352012000500004.
The pyometra in 31 bitches of different breeds and ages, 25 with pyometra cervix open and 6 closed cervix was analyzed. After the ovariohysterectomy procedure, samples were collected from the cervix and uterus to evaluate immunohistochemistry. For immunohistochemical evaluation estrogen receptors α and β, progesterone and collagen I and III were analyzed. Immunostainings were performed in different regions of the cervix such as glandular epithelium, luminal epithelium and glandular stroma, as well as in different regions of the uterus, such as glandular epithelium and glandular stroma. The immunostainings for collagen I and III were performed in muscular and glandular regions of the cervix and uterus. The concentration the progesterone receptors were elevated in bitches from the closed pyometra.
Keywords : bitches; immunohistochemistry; pyometra.