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Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Print version ISSN 0102-0935


MASCARENHAS, R.M.; XAVIER, P.R.  and  MARQUES JUNIOR, A.P.. Individual ejaculates and pooled semen: differences under experimental conditions. Arq. Bras. Med. Vet. Zootec. [online]. 2014, vol.66, n.1, pp.22-26. ISSN 0102-0935.

Individual ejaculates and pooled dog semen submitted to two treatments of medium exchange were evaluated. Semen was collected from six dogs, as individual ejaculates and pooled semen, diluted in a 1:1 ratio in Tris-Yolk medium, centrifuged at 500g/10min and ressuspended to the final concentration of 50x106 sptz/mL. The samples were cooled at rates of 0.26 o C/min between 37 and 16 o C, and 0.08ºC/min from 16 to 8ºC, and then kept in a refrigerator for 14 days. In Treatement 1 medium was exchanged every six days and in Treatment 2 after twelve days. The cooled samples were evaluated every 48 hours for sperm motility using the Sperm Calss Analyser® (SCA), and membrane integrity with hiposmotic swelling test and PI/CFDA stain. Pooled semen was easier to handle, mainly considering the decreased work due to volume. When submitted to medium exchange, pooled semen behaved similarly to individual ejaculates; however, results obtained from individual ejaculates showed differences between treatments, which were not apparent in pooled semen results.

Keywords : dog; pooled semen; cryopreservation.

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