SciELO - Scientific Electronic Library Online

 
vol.66 issue1Mitral flow propagation velocity in non-sedated healthy catsImmunohistochemistry analysis to confirm cases of cutaneous pythiosis in horses diagnosed by means of clinic characterization and histopathologic evaluation author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Print version ISSN 0102-0935

Abstract

MASCARENHAS, R.M.; XAVIER, P.R.  and  MARQUES JUNIOR, A.P.. Individual ejaculates and pooled semen: differences under experimental conditions. Arq. Bras. Med. Vet. Zootec. [online]. 2014, vol.66, n.1, pp.22-26. ISSN 0102-0935.  http://dx.doi.org/10.1590/S0102-09352014000100004.

Individual ejaculates and pooled dog semen submitted to two treatments of medium exchange were evaluated. Semen was collected from six dogs, as individual ejaculates and pooled semen, diluted in a 1:1 ratio in Tris-Yolk medium, centrifuged at 500g/10min and ressuspended to the final concentration of 50x106 sptz/mL. The samples were cooled at rates of 0.26 o C/min between 37 and 16 o C, and 0.08ºC/min from 16 to 8ºC, and then kept in a refrigerator for 14 days. In Treatement 1 medium was exchanged every six days and in Treatment 2 after twelve days. The cooled samples were evaluated every 48 hours for sperm motility using the Sperm Calss Analyser® (SCA), and membrane integrity with hiposmotic swelling test and PI/CFDA stain. Pooled semen was easier to handle, mainly considering the decreased work due to volume. When submitted to medium exchange, pooled semen behaved similarly to individual ejaculates; however, results obtained from individual ejaculates showed differences between treatments, which were not apparent in pooled semen results.

Keywords : dog; pooled semen; cryopreservation.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License