SciELO - Scientific Electronic Library Online

vol.24 número1Lycopodiaceae no Parque Nacional do Itatiaia, RJ e MG, BrasilDiversidade polínica das Mimosoideae (Leguminosae) ocorrentes em uma área de caatinga, Pernambuco, Brasil índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados




Links relacionados


Acta Botanica Brasilica

versão impressa ISSN 0102-3306


SILVA, Janaína de Oliveira Costa et al. Gibberellic acid (GA3) and anatomy of the leaf epidermises to detect somaclonal variants of the banana tree Musa sp. Colla cv. Prata-anã (Musaceae). Acta Bot. Bras. [online]. 2010, vol.24, n.1, pp.47-52. ISSN 0102-3306.

Micropropagation of Musa plants is applied on a wide scale in order to obtain plantlets with high sanitary quality. In vitro cultivation may lead to somaclonal variation that can cause harm the producers. Currently, differentiation of somaclonal variants is possible only in the field based on the exaggerated growth of the plants. Mutants for gibberellin production are dwarfed and this mutation is reverted by applying gibberellic acid. The anatomy of plants cultivated in vitro and ex vitro can give information to improve micropropagation techniques. The objective of this work was to differentiate normal plants from variant ones by anatomic structures. For this propose, leaves of banana plants 'Prata-anã' cultivated in vitro, during the acclimatization phase and in the field after the visual detection of somaclonal variation, were used. Paradermic sections were produced to evaluate the stomata complex. Transverse sections were done to measure adaxial and abaxial epidermal-face thickness, chlorophyll parenchyma andmidrib. Structural differences were detected in plants during developmental phases. Moreover, in this work it was possible to determine anatomical markers to distinguish normal from variant plants based on midrib diameter and inferior hypodermis.

Palavras-chave : in vitro culture; Anatomic markers; Phenotypic variation.

        · resumo em Português     · texto em Português     · Português ( pdf )


Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons