The genus Psychotria comprises approximately 2,000 species that are mostly shrubs distributed in forests of the tropics and subtropics. Within the Rubiaceae, Psychotria is well known for its species that produce bioactive alkaloids and for its taxonomic complexity. The anatomical and micromorphological characters of the leaves of P. hoffmannseggiana, P. carthagenensis, P. deflexa, P. glaziovii, P. leiocarpa, P. racemosa, and P. vellosiana were investigated and described to understand the interspecific variation of these species, which will contribute to the systematics of the genus. Principal component analysis (PCA) and unweighted pair group method analysis (UPGMA) indicated four groups based on the presence or absence of some of the characters studied. Dorsiventral mesophyll, hypostomatic leaves, paracytic stomata, organization of the vascular system of the petiole and midrib in an arc and the presence of accessory bundles are common in the species examined and are typical characteristics of the Rubiaceae. The presence of styloid crystals and alkaloids are considered diagnostic characters of Psychotria. Other characteristics used to separate species in the genus are the petiole contour, cuticle ornamentation, and presence of trichomes. The results of the UPGMA cluster analyses indicated that there is similarity between Psychotria (subg. Heteropsychotria) and Palicourea species.
leaf anatomy; cluster analysis; principal component analysis; systematic anatomy; domatia; leaf surface morphology
