Revista Brasileira de Ortopedia
versión impresa ISSN 0102-3616
CASTIGLIA, Marcello Teixeira; SILVA, Juliano Voltarelli F. da; THOMAZINI, José Antonio y VOLPON, José Batista. Effects of chemical processing and oxide ethylene sterilization on cortical and cancellous rat bone: a light and electron scanning microscopy study. Rev. bras. ortop. [online]. 2009, vol.44, n.1, pp. 32-39. ISSN 0102-3616. http://dx.doi.org/10.1590/S0102-36162009000100005.
OBJECTIVE: To evaluate, under microscopic examination, the structural changes displayed by the trabecular and cortical bones after being processed chemically and sterilized by ethylene oxide. METHODS: Samples of cancellous and cortical bones obtained from young female albinus rats (Wistar) were assigned to four groups according to the type of treatment: Group I-drying; Group II-drying and ethylene oxide sterilization; III-chemical treatment; IV-chemical treatment and ethylene oxide sterilization. Half of this material was analyzed under ordinary light microscope and the other half using scanning electron microscopy. RESULTS: In all the samples, regardless the group, there was good preservation of the general morphology. For samples submitted to the chemical processing there was better preservation of the cellular content, whereas there was amalgamation of the fibres when ethylene oxide was used. CONCLUSION: Treatment with ethylene oxide caused amalgamation of the fibers, possibly because of heating and the chemical treatment contributed to a better cellular preservation of the osseous structure.
Palabras llave : Bone transplantation; Ethylene oxide; Microscopy, electron; Sterilization; Rats.