Revista Brasileira de Farmacognosia
versión impresa ISSN 0102-695X
ANDRADE, Cláudia Alexandra de et al. Determination of the phenolic content and evaluation of the antioxidant activity of Acacia podalyriifolia A. Cunn. ex G. Don, Leguminosae-mimosoideae. Rev. bras. farmacogn. [online]. 2007, vol.17, n.2, pp. 231-235. ISSN 0102-695X. http://dx.doi.org/10.1590/S0102-695X2007000200017.
Acacia species are usually used as treatment for several diseases. Chemotaxonomic studies have related the Acacia genus as a source of phenolic compounds, which present several biological properties. This work emphasizes the antioxidant activity of Acacia podalyriifolia. This plant is natural from Australia and vastly cultivated in the South of Brazil as ornamental feature. The plant material (flowers) was shadow-dried and submitted to the extraction with ethanol, followed by partition with hexane, dichloromethane and ethyl acetate. The phytochemical screening performed in the ethanol crude extract and in its fractions showed the presence of phenolic compounds and flavonoids. Determination of the phenolic content was performed with the Folin Ciocalteau reagent. It was found out that the ethyl acetate fraction (FAE) was the one that displayed the biggest concentration (338,5 mg of gallic acid/g of FAE). In the evaluation of the radical scavenging activity, using the free radical DPPH, the ethyl acetate fraction showed the highest antioxidant activity (IC50 = 3,22 µg/mL from FAE).
Palabras llave : Acacia podalyriifolia; antioxidant; phenolic compounds.