Revista Brasileira de Farmacognosia
On-line version ISSN 1981-528X
ALVES, Tânia Maria Almeida et al. Morphological, anatomical, macro and micromolecular markers for Solanum cernuum identification. Rev. bras. farmacogn. [online]. 2007, vol.17, n.4, pp. 542-548. ISSN 1981-528X. http://dx.doi.org/10.1590/S0102-695X2007000400012.
The plants consumed as remedy by the population may have imprecise taxonomical identification. If these plants are used for the production of phytomedicines such misidentification may affect the quality of the product. Hereby, we describe markers for identification of the entire plant or grounded plant material or the crude extract of Solanum cernuum Vell. (Solanaceae). Specimens from four localities were collected, analyzed and compared. Morphological characters were used to identify the plant when it is not grounded or extracted. However, when the plant material is grounded, the set of trichomes may be used as anatomical marker. The region ITS1, 5.8S and ITS2 of the nuclear ribosomal DNA was cloned and sequenced. The sequence, with length of about 600 base pairs, being 48.1% AT , was deposited in GenBank under the accession number DQ837371. Once this sequence is specific to S. cernuum, it was used as marker for this species. For the crude extract, chromatographic profiles of the leaves extracts were obtained by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Two flavonoids were isolated and identified as quercitrin and afzelin. So, this study presents morphological, anatomical, macro and micromolecular markers to identify S. cernuum.
Keywords : Solanum cernuum; Solanaceae; trichomes; quercitrin; afzelin; ITS rDNA.