Revista Brasileira de Farmacognosia
Print version ISSN 0102-695X
SIQUEIRA, Ezequias Pessoa de et al. Leishmanicidal activities of the extract from Blepharocalyx salicifolius (Kunth) O. Berg, Myrtaceae. Rev. bras. farmacogn. [online]. 2010, vol.20, n.3, pp. 416-421. ISSN 0102-695X. http://dx.doi.org/10.1590/S0102-695X2010000300020.
Crude ethanolic extracts from Blepharocalyx salicifolius (Kunth) O. Berg, Myrtaceae, was fractioned by Gel Permeation Chromatography, using SephadexTM LH-20 gel. Sixteen fractions were obtained and were supplied to cytotoxicity in vitro assay against Leishmania (L.) amazonensis amastigota cells. It was observed eight cytotoxic fractions against Leishmania (L.) amazonensis amastigota cells at range of 19 to 29 µg.mL-1. Two of them were not citotoxic against human peripheral blood mononuclear cell, with a great potential to isolation of more selective leishmanicidal substances.
Keywords : Blepharocalyx salicifolius; Myrtaceae; Leishmania (Leishmania) amazonensis; gel permeation chromatography; citotoxicity.