SciELO - Scientific Electronic Library Online

vol.21 issue3Influence of gamma radiation on the antimicrobial activity of crude extracts of Anacardium occidentale L., Anacardiaceae, rich in tanninsIn vitro cytotoxic activity of Brazilian Middle West plant extracts author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista Brasileira de Farmacognosia

Print version ISSN 0102-695X


SOUZA, Stefânia P. de; PEREIRA, Luciana L. S.; SOUZA, Alline A.  and  SANTOS, Custódio D. dos. Inhibition of pancreatic lipase by extracts of Baccharis trimera (Less.) DC., Asteraceae: evaluation of antinutrients and effect on glycosidases. Rev. bras. farmacogn. [online]. 2011, vol.21, n.3, pp.450-455.  Epub Apr 01, 2011. ISSN 0102-695X.

In order to confirm the traditional use of Baccharis trimera (Less.) DC., Asteraceae, for the reduction of weight, plant extracts were evaluated on the activity of pancreatic lipase (PL), an enzyme responsible for hydrolysis of triacylglycerols in the diet for its subsequent absorption. The aqueous and infused extracts did not show inhibitory activity on the PL, the ethanol inhibited 16% (66 ALI/g) and methanol extract inhibited 78% (241 ALI/g). The methanol extract of B. trimera (MEB) was subjected to a wash with decreasing solvent polarity (n-hexane, ethyl acetate and methanol) and only the methanol fraction inhibited the lipase by 72% (230 ALI/g). We evaluated the MEB and infused inhibitory activity on the enzymes α-amylase and α and β-glycosidases. The α-amylase was not inhibited by any of the extracts, the enzyme α-glucosidase was inhibited by both extracts in the same proportion (46.9±0.1) and β-glucosidase was inhibited by 73% by the methanol extract and 65% by the infused. We also evaluated the presence of anti-nutrients. We detected the presence of saponins, polyphenols and trypsin inhibitors in the two samples. Tests performed in vivo can assess at which therapeutic concentration the presence of these anti-nutrients can be harmful to health.

Keywords : obesity; pancreatic lipase; Baccharis trimera; anti-nutrients.

        · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License