Revista Brasileira de Farmacognosia
Print version ISSN 0102-695X
MOURA, Túlio Flávio A. L.; RAFﬁN, Fernanda N. and SANTOS, Ana Lourdes R.. Evaluation of a preservative system in a gel containing hydroalcoholic extract of Schinus terebinthifolius. Rev. bras. farmacogn. [online]. 2011, vol.21, n.3, pp. 532-536. Epub June 17, 2011. ISSN 0102-695X. http://dx.doi.org/10.1590/S0102-695X2011005000102.
Currently, microbial contamination is one of the major problems faced by the phytomedicine industry with respect to the quality of the raw materials. The objective of this study was to evaluate the effectiveness of a preservative system in a formulation with hydrogel containing hydroalcoholic extract of Schinus terebinthifolius Raddi, Anacardiaceae ("aroeira" or "Brazilian Peppertree"), through the challenge test. The extracts were prepared by maceration at a ratio of 1:10 plant/solvent in 40% alcohol. Gel samples were artificially contaminated with separate inocula of Aspergillus niger, Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa and the number of viable microorganisms determined in triplicate by the "pour plate" method for counting colonies at 0, 24 and 48h, 7, 14, 21 and 28 days. The addition of the preservatives (methyl and propylparaben) in the "aroeira" gel proved to be effective against the studied species when the samples were evaluated using the challenge test. According to the criterion A of the European Pharmacopoeia, it was verified that the hydrogel product showed good conservation in a 28 days period.
Keywords : challenge test; preservative system; Schinus terebinthifolius.