Revista Brasileira de Farmacognosia
Print version ISSN 0102-695X
MOTTA, Lucimar B. et al. Constituents and antiproliferative activity of extracts from leaves of Croton macrobothrys. Rev. bras. farmacogn. [online]. 2011, vol.21, n.6, pp.972-977. Epub Sep 23, 2011. ISSN 0102-695X. http://dx.doi.org/10.1590/S0102-695X2011005000174.
Croton macrobothrys Baill, Euphorbiaceae, is a tree from the Atlantic Forest in Southern Brazil, used in traditional medicine and popularly known as "dragon's blood" and "pau-sangue". Leaf n-hexane, dichloromethane and methanol extracts were analyzed by GC/MS and evaluated for their in vitro antiproliferative activity on cell lines 786-0 (kidney), HT-29 (colon), K562 (leukemia), NCI-ADR/RES (drug resistant ovary), NCI-H460 (lung), MCF-7 (mammary), PC-3 (prostate), OVCAR-3 (ovary), U251 (glioma) and UACC-62 (melanoma). The dicloromethane extract exhibited activity against all cell lines at the concentration 25 µg/mL, in particular on cell lines NCI-H460 (GI50 0.33 μg/mL) and K5662 (GI50 0.91 μg/mL). Relevant constituents in dichloromethane extract are the alkaloids corydine and salutaridine, as well as the diterpenes geranylgeraniol and crotonin-derived clerodanes.
Keywords : alkaloids; antiproliferative activity; diterpenes; medicinal plants.