Revista Brasileira de Farmacognosia
Print version ISSN 0102-695X
COSTA JUNIOR, Joaquim S. da et al. Evaluation of effects of dichloromethane fraction from Platonia insignis on pilocarpine-induced seizures. Rev. bras. farmacogn. [online]. 2011, vol.21, n.6, pp. 1104-1110. Epub Sep 09, 2011. ISSN 0102-695X. http://dx.doi.org/10.1590/S0102-695X2011005000163.
The objective of present study was to evaluate the antioxidant and anticonvulsant activities of dichloromethane fraction (DMF) from Platonia insignis Mart., Clusiaceae. The DMF from P. insignis (2 mg/kg) was tested by intraperitoneal (i.p.) to evaluate effects on lipid peroxidation level, nitrite formation, as well as on locomotor and anticonvulsant activities. Wistar rats were treated with, (saline/Tween 80 0.5%, i.p., control group), DMF (2 mg/kg, i.p., DMF group), pilocarpine (400 mg/kg, i.p., P400 group), or the combination of DMF (2 mg/kg, i.p.) and pilocarpine (400 mg/kg, i.p., DMF plus P400). After the treatments all groups were observed for 24 h. In P400 group rats there was a decrease in the motor activity when compared with control group. In DMF plus P400 co-administered rats was observed an increase in motor activity when compared with P400 group. In P400 group rats there was a significant increase in lipid peroxidation and nitrite levels. In DMF plus P400 co-administered rats, antioxidant treatment significantly reduced the lipid peroxidation level and nitrite content after seizures. Previous findings strongly support the hypothesis that oxidative stress occurs in rat striatum during pilocarpine-induced seizures, and our results imply that strong neuprotective effect on this brain region could be achieved using DMF from P. insignis.
Keywords : lipid peroxidation; nitrite; pilocarpine; P. insignis; seizures.