Abstract

WOLLMANN, Luciana Cristina Ferretti de Nazareno; LAURINDO, Carlos A. H; COSTA, Francisco Diniz Affonso da  and  MORENO, Andréa Novais. Effects of cryopreservation and/or decellularization on extracellular matrix of porcine valves. Rev Bras Cir Cardiovasc [online]. 2011, vol.26, n.3, pp. 490-496. ISSN 0102-7638.  http://dx.doi.org/10.5935/1678-9741.20110029.

The objective of this study was to evaluate the morphology of decelluarized and/or cryopreserved porcine pulmonary valves, to determine a solution capable of completely remove the cells without damaging the extracellular matrix. Porcine pulmonary valves were incubated for 24 hs in sodium deoxicholate 1% or sodium dodecyl sulfate 0.1 and 0.3%, with or without associated cryopreservation. Evaluation was done with optical microscopy (Hematoxilin-Eosin, Acetic Orcein and Gomori) and with morphometric analysis. The effectiviness of the solutions was variable, but the best results were obtained with the sodium dodecyl sulfate solution 0.1%.

Keywords : Tissue Engineering; Extracellular Matrix; Biotechnology; Morphology.