SciELO - Scientific Electronic Library Online

vol.26 issue3Lymphoscintigraphic changes after harvesting of the saphenous vein for coronary artery bypass graftMultiple left atrial myxoma: case report author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Journal of Cardiovascular Surgery

Print version ISSN 0102-7638


WOLLMANN, Luciana Cristina Ferretti de Nazareno; LAURINDO, Carlos A. H; COSTA, Francisco Diniz Affonso da  and  MORENO, Andréa Novais. Effects of cryopreservation and/or decellularization on extracellular matrix of porcine valves. Rev Bras Cir Cardiovasc [online]. 2011, vol.26, n.3, pp.490-496. ISSN 0102-7638.

The objective of this study was to evaluate the morphology of decelluarized and/or cryopreserved porcine pulmonary valves, to determine a solution capable of completely remove the cells without damaging the extracellular matrix. Porcine pulmonary valves were incubated for 24 hs in sodium deoxicholate 1% or sodium dodecyl sulfate 0.1 and 0.3%, with or without associated cryopreservation. Evaluation was done with optical microscopy (Hematoxilin-Eosin, Acetic Orcein and Gomori) and with morphometric analysis. The effectiviness of the solutions was variable, but the best results were obtained with the sodium dodecyl sulfate solution 0.1%.

Keywords : Tissue Engineering; Extracellular Matrix; Biotechnology; Morphology.

        · abstract in Portuguese     · text in English | Portuguese     · English ( pdf epdf ) | Portuguese ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License