Acta Cirurgica Brasileira
On-line version ISSN 1678-2674
GRAGNANI, Alfredo; MORGAN, Jeffrey R. and FERREIRA, Lydia Masako. Experimental model of cultured skin graft. Acta Cir. Bras. [online]. 2004, vol.19, suppl.1, pp. 4-10. ISSN 1678-2674. http://dx.doi.org/10.1590/S0102-86502004000700003.
One of the most used animal models of cultured keratinocytes autografting is based on xenografting of human keratinocytes to the rat or athymic mice, immunological neutral recipient that acts as biological carrier. It could be studied in this model many facts that occur after transplant without the ethical aspect in the clinical study. The proposition of the experimental model is related to the sequence of the total or partial skin transplant, as autografting or xenografting, cultured or not, to the back of athymic mice. The model presents the possibility of study in vivo athymic animal, when the in vivo study in anima nobili is not ethical. It permits the xenografting evaluation of cultured cells graft or of the genetically modified cells and of the association of the cultured cells and the dermal substitutes, the composite grafts, and of the autografting.
Keywords : Keratinocytes; Dermis; Skin transplantation; Cells, Cultured; Athymic mice.