Acta Cirurgica Brasileira
On-line version ISSN 1678-2674
KEIRA, Sidney Mamoru et al. Experimental model for fibroblast culture. Acta Cir. Bras. [online]. 2004, vol.19, suppl.1, pp. 11-16. ISSN 1678-2674. http://dx.doi.org/10.1590/S0102-86502004000700004.
The use of cell culture methods in Plastic Surgery opened a new horizon in the research of cellular mechanisms of proliferation and biosynthesis functions. Several types of cells have been investigated in the cutaneous compartment. Keratinocytes and fibroblasts have been studied aiming the possibility of developing biomaterial for skin substitution. The present study describes the standardization for the development of fibroblast primary culture, its utilization in experiments and its storage.
Keywords : Cell culture; Fibroblasts.