Acta Cirurgica Brasileira
On-line version ISSN 1678-2674
KEIRA, Sidney Mamoru et al. Experimental model for establishment of hypoxia in 75 cm2 culture flasks. Acta Cir. Bras. [online]. 2004, vol.19, suppl.1, pp. 23-27. ISSN 1678-2674. http://dx.doi.org/10.1590/S0102-86502004000700006.
In Plastic Surgery, cell culture represents the perspective of studying cellular mechanisms that guide the healing process of several tissues. Some steps of the healing process depend on physical factors as the tissular partial pressure of O2. In cell culture, it is possible to submit cells to hypoxic enviroment. The present study reports an alternative method at low cost for the establishment of hypoxic environment in cell culture flasks.
Keywords : Cells, cultured; Hypoxia.