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Acta Cirúrgica Brasileira

versão impressa ISSN 0102-8650versão On-line ISSN 1678-2674

Resumo

BRITO, Nara Macedo Botelho; TORRES, Inêz Ohashi; SILVEIRA, Edvaldo Lima  e  SANTOS, Lorena Lira Tavares dos. Study about the feasibility of ovarian graft in rats' omentum pouch, using cyclosporine A. Acta Cir. Bras. [online]. 2005, vol.20, n.2, pp.174-179. ISSN 1678-2674.  https://doi.org/10.1590/S0102-86502005000200013.

PURPOSE: To study comparatively ovarian autograft and heterograft in rats' omentum pouch, using Cyclosporine A. METHODS: In the present study 20 rats, distributed equally in four groups, had been used: Control Group (rats submitted to ooforectomy); Group A (ovarian autograft); group H (ovarian heterograft); e group C (ovarian heterograft and treatment with Cyclosporine A). All animals had been submitted to a bilateral ooforectomy. One of the removed ovaries had been individualized and grafted in Greater Omentum. The animals from group C received 10mg/kg/dia of cyclosporine subcutaneously during the period of the experiment. All animals had been submitted to a new surgical intervention after a period of 33 days for identification of the receiving bedstead and withdrawal of the grafted ovary, beyond the uterus and the vagina, followed of euthanasia. The parts had been submitted to the histological process of routine. RESULTS: All groups presented vaginal and uterine epithelium with one or two rows of simple cubical or cylindrical cells, spherical and central nucleus. It also had great inflammatory reaction in all grafts, with necrosis areas. At the 20th day of the experiment, one of the animals of group C died. CONCLUSION: It was concluded that the graft of the entire ovary in Greater Omentum of rats in this sample, independently of the use of Cyclosporine, did not revealed viable.

Palavras-chave : Graft; Ovary; Survival Peritoneal Cavity; Cyclosporine; Rats.

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