Acta Cirurgica Brasileira
On-line version ISSN 1678-2674
PRADO, Rodrigo Paschoal et al. Experimental model for low level laser therapy on ischemic random skin flap in rats. Acta Cir. Bras. [online]. 2006, vol.21, n.4, pp. 258-262. ISSN 1678-2674. http://dx.doi.org/10.1590/S0102-86502006000400013.
PURPOSE: To develop an experimental model to be used in the study of low level Laser therapy on viability of random skin flap in rats. METHODS: The sample was 24 Wistar-EPM rats. The random skin flap measured 10 x 4 cm and a plastic sheet was interposed between the flap and donor site. Group 1 (control) underwent sham irradiation with diode laser (830 nm). Group 2 was submitted to laser irradiation with diode laser (830 nm). The animals were submitted to Laser therapy with 36 J/cm2 energy density (72 seconds) immediately after the surgery and on the four subsequent days. The probe was usually held in contact with the skin flap surface on a point at 2.5 cm cranial from the flap base. On the seventh postoperative day, the percentage of necrotic area was measured and calculated. RESULTS: Group 1 reached an average necrotic area of 48.86%, Group 2 - 23.14%. After the statistic analysis, compared with the control group, Group 2 showed a statistically significant increase in survival area (p<0.001). CONCLUSION: The experimental model proved to be reliable to be used in the study of effects of low level laser therapy in random skin flap in rats.
Keywords : Laser Therapy, Low-Level; Surgical Flaps; Necrosis; Rats.