Acta Cirurgica Brasileira
On-line version ISSN 1678-2674
NEGREIROS, Róbson Miguel de Araújo et al. Experimental splenectomies and malaria in mice. Acta Cir. Bras. [online]. 2009, vol.24, n.6, pp. 437-441. ISSN 1678-2674. http://dx.doi.org/10.1590/S0102-86502009000600003.
PURPOSE: To evaluate the importance of spleen in malaric infection in murino model, comparing the parasitemia and the titles of imunoglobulins in the different groups. METHODS: It was used female mice non-isogenic, in inoculated with Plasmodium berghei, cepa ANKA, intraperitoneally. The parasitemia was analyzed in 23rd, 25th, 27th and 32nd day of the experiment, being the stained blood' exam colored by Giemsa. The titles of the total serum immunoglobulins IgM and IgG were analyzed by Dot-ELISA technique, at 6th, 22nd and 32nd day, when the animals were sacrificed. RESULTS: The parasitemia was gradual in all the inoculated groups. In the end of the experiment, the animals with partial parasitemia present superior parasitemia, but next to the non-splenectomized, while the asplenics present difference bigger than the double. The levels of total serum IgM and IgG didin´t have significant changes with the removal partial or total splenic. CONCLUSION: The techniques conservatives in splenic trauma are possible and necessary. The importance of remaining spleen in the clearance of red blood cells parasitized by Plasmodium berghei showed being efficient, in order to avoid serious complications resulting of the malaria in mice.
Keywords : Splenectomy; Malaria; Sepsis; Infection; Mice.