Acta Cirurgica Brasileira
versión On-line ISSN 1678-2674
OLIVEIRA, Andrea Fernandes de et al. Differential diagnosis in primary and metastatic cutaneous melanoma by FT-Raman spectroscopy. Acta Cir. Bras. [online]. 2010, vol.25, n.5, pp. 434-439. ISSN 1678-2674. http://dx.doi.org/10.1590/S0102-86502010000500009.
PURPOSE: To qualify the FT-Raman spectral data of primary and metastatic cutaneous melanoma in order to obtain a differential diagnosis. METHODS: Ten normal human skin samples without any clinical or histopathological alterations, ten cutaneous melanoma fragments, and nine lymph node metastasis samples were used; 105, 140 and 126 spectra were obtained respectively. Each sample was divided into 2 or 3 fragments of approximately 2 mm3 and positioned in the Raman spectrometer sample holder in order to obtain the spectra; a monochrome laser light Nd:YAG at 1064 nm was used to excite the inelastic effect. RESULTS: To differentiate the three histopathological groups according to their characteristics extracted from the spectra, data discriminative analysis was undertaken. Phenylalanine, DNA, and Amide-I spectral variables stood out in the differentiation of the three groups. The percentages of correctly classified groups based on Phenylalanine, DNA, and Amide-I spectral features was 93.1%. CONCLUSION: FT-Raman spectroscopy is capable of differentiating melanoma from its metastasis, as well as from normal skin.
Palabras llave : Melanoma; Spectrum Analysis, Raman; Diagnosis; Biopsy.