Acta Cirurgica Brasileira
On-line version ISSN 1678-2674
MONTEIRO, Betânia Souza et al. Association of mesenchymal stem cells with platelet rich plasma on the repair of critical calvarial defects in mice. Acta Cir. Bras. [online]. 2012, vol.27, n.3, pp. 201-209. ISSN 1678-2674. http://dx.doi.org/10.1590/S0102-86502012000300001.
PURPOSE: To evaluate the effects of mesenchymal stem cells (MSC) from eight mice C57BL/6 gfp+ bone marrows expanded in cultures associated with platelets rich plasma (PRP) deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice C57BL/6. METHODS: The animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. Control group did not receive treatment and the treated group received a MSC pellet containing 1.0 x 107 cells/mL associated with 50.0µL of plasma gel containing 1.0 x 109 autologous platelets within the defect. RESULTS: In the treated group was observed process of angiogenesis and bone repair better than control group. CONCLUSION: Mesenchymal stem cells derived from bone marrow of C57BL/6 gfp+ mice associated with PRP gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.
Keywords : Mesenchymal Stem Cells; Platelet-Rich Plasma; Tissue Therapy; Skull; Mice.