SciELO - Scientific Electronic Library Online

 
vol.27 issue9Protective effect of N-acetylcysteine on kidney as a remote organ after skeletal muscle ischemia-reperfusionUse of 2-octyl cyanoacrylate adhesive in rat liver induced lesion author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Acta Cirurgica Brasileira

On-line version ISSN 1678-2674

Abstract

TAHA, Murched Omar et al. L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury. Acta Cir. Bras. [online]. 2012, vol.27, n.9, pp.616-623. ISSN 1678-2674.  https://doi.org/10.1590/S0102-86502012000900005.

PURPOSE: To investigate the effects of intravenous L-arginine (LG) infusion on liver morphology, function and proinflammatory response of cytokines during the early phase of ischemia-reperfusion injury (IRI). METHODS: Thirty rabbits were subjected to 60 minutes of hepatic ischemia and 120 minutes of reperfusion. An intravenous injection of saline or L-arginine was administered five minutes before the ischemia and five minutes before initiating the reperfusion and at the 55th and 115th minutes after the ischemia. Samples were collected for histological analysis of the liver and measurements of the serum AST, ALT and LDH and the cytokines IL-6 and TNF-alpha. RESULTS: It was observed a significant reduction of sinusoidal congestion, cytoplasmic vacuolization, infiltration of polymorphonuclear leukocyte, nuclear pyknosis, necrosis and steatosis in liver tissue, as well as AST, ALT and LDH after injection of LG in the ischemia (p <0.001). Lower levels of IL-6 and TNF-alpha were associated with LG infusion during ischemia. Higher levels these proteins were observed in animals receiving LG during reperfusion. CONCLUSION: L-arginine protects the liver against ischemia/reperfusion injury, mainly when is administered during the ischemic phase.

Keywords : Nitric Oxide; Arginine; Warm Ischemia; Cytokines; Antioxidants; Interleukin-6; Liver; Rabitts.

        · abstract in Portuguese     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License