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Acta Cirurgica Brasileira

versão On-line ISSN 1678-2674


RAYMUNDO, Erica Calcagno et al. Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin. Acta Cir. Bras. [online]. 2014, vol.29, n.4, pp.224-230. ISSN 1678-2674.


To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin.


Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB).


In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups.


Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin.

Palavras-chave : Carbon Dioxide; Substance P; Calcitonin Gene-Related Peptide; Neurogenic Inflammation; Skin; Rats.

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