Lipoprotein lipase gene-deficient mice with hypertriglyceridaemia associated with acute pancreatitis

Tang, Maochun; Zong, Pengfei; Zhang, Ting; Wang, Dongyan; Wang, Yuhui; Zhao, Yan

doi:10.1590/S0102-865020160100000003

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ORIGINAL ARTICLES • Acta Cir. Bras. 31 (10) • Oct 2016 • https://doi.org/10.1590/S0102-865020160100000003 copy

Lipoprotein lipase gene-deficient mice with hypertriglyceridaemia associated with acute pancreatitis^¹ 1 Research performed at Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

PURPOSE:

To investigate the severity of pancreatitis in lipoprotein lipase (LPL)-deficient hypertriglyceridaemic (HTG) heterozygous mice and to establish an experimental animal model for HTG pancreatitis study.

METHODS:

LPL-deficient HTG heterozygous mice were rescued by somatic gene transfer and mated with wild-type mice. The plasma amylase, triglyceride, and pathologic changes in the pancreas of the LPL-deficient HTG heterozygous mice were compared with those of wild-type mice to assess the severity of pancreatitis. In addition, acute pancreatitis (AP) was induced by caerulein (50 µg/kg) for further assessment.

RESULTS:

The levels of plasma amylase and triglyceride were significantly higher in the LPL-deficient HTG heterozygous mice. According to the pancreatic histopathologic scores, the LPL-deficient HTG heterozygous mice showed more severe pathologic damage than the wild-type mice.

CONCLUSIONS:

Lipoprotein lipase deficient heterozygous mice developed severe caerulein-induced pancreatitis. In addition, their high triglyceride levels were stable. Therefore, LPL-deficient HTG heterozygous mice are a useful experimental model for studying HTG pancreatitis.

Key words:
Hypertriglyceridemia; Pancreatitis; Lipoprotein Lipase; Mice

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[1] Correspondence: Yan Zhao Department of Gastroenterology Shanghai Tenth People's Hospital, Tongji University School of Medicine No. 301 Yanchangzhong Road Shanghai 200072 China yanzhaocn@yeah.net

Normal blood lipid group (wild type) (n=28)	Hyperlipidemia group (heterozygote) (n=26)
E: control group of wild type with normal saline for 12 h (n=7)	A: control group of heterozygote animals with normal saline for 12 h (n=6)
F: control group of wild type with normal saline for 24 h (n=7)	B: control group of heterozygote animals with normal saline for 24 h (n=6)
G: experimental group of wild type animals with intraperitoneal administration of caerulin for 12 h (n=7)	C: experimental group of heterozygote animals with intraperitoneal administration of caerulin for 12 h (n=7)
H: experimental group of wild type animals with intraperitoneal administration of caerulin for 24 h (n=7)	D: experimental group of heterozygote animals with intraperitoneal administration of caerulin for 24 h (n=7)

Heterozygote				Wild type
	Control group 12 h	Control group 24 h	Experimental group 12 h	Experimental group 24 h	Control group 12 h	Control group 24 h	Experimental group 12 h	Experimental group 24 h
Amylase (U/L) TG (Mmol/L)	471.29± 22.74 3.547± 0.27	549.98± 93.49 3.55± 0.27	3685.06± 483.98*# 3.548± 0.267	3019.81± 643.36*# 3.557± 0.321	358.33± 42.72 0.937± 0.183	385.76± 37.49 0.884± 0.149	2500.89± 409.59* 0.92± 0.107	2090.67± 700.95* 0.921± 0.074

Test groups	Time point	Edema	Necrosis	Bleeding	Inflammatory cell infiltration
Heterozygote control group	12 h	0.80± 0.27	1.78± 0.43	0.80± 0.25	0.10± 0.22
	24 h	0.80± 0.26	1.77± 0.42*	0.81± 0.23	0.10± 0.22
Heterozygote experimental group	12 h	3.94± 0.21*	3.94± 0.21*	1.84± 0.25*	1.84± 0.25*
	24 h	3.57± 0.37*	2.05± 0.87	1.13± 0.52	1.18± 0.66*
Wild type control group	12 h	0.50± 0.50	0.50± 0.35	0.50± 0.50	0.10± 0.22
	24 h	0.50± 0.50	0.50± 0.35	0.50± 0.50	0.10± 0.22
Wild type experimental group	12 h	3.06± 0.01	2.52± 0.51	0.46± 0.22	0.58± 0.38
	24 h	2.52± 0.01	1.41± 0.22	0.58± 0.44	0.27± 0.22

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Lipoprotein lipase gene-deficient mice with hypertriglyceridaemia associated with acute pancreatitis1 1 Research performed at Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

ABSTRACT

PURPOSE:

METHODS:

RESULTS:

CONCLUSIONS:

Lipoprotein lipase gene-deficient mice with hypertriglyceridaemia associated with acute pancreatitis^¹ 1 Research performed at Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.