Characterization of an experimental model of progressive renal disease in rats

Eighty male Wistar rats were divided into four experimental groups (n=20/group): sham surgery (control group), progressive degrees of nephrectomy leading to mild uremia (group 1), moderate uremia (group 2) and severe uremia (group 3). Ten animals of each group were followed for two or four weeks. At the end, blood and 24-hour urine samples were collected to determine renal function parameters. Urine output and water and food intake were daily monitored.

RESULTS:

In rats of group 1, serum levels of creatinine and urea and microalbuminuria were increased, while reduced creatinine clearance (p<0.05, compared with control group), without changing blood pressure. Animals of group 2 had more accentuated alterations: increases in urinary output, blood pressure, serum concentrations of urea, creatinine, sodium, potassium, and in microalbuminuria, and reduction of creatinine clearance (p<0.05). Group 3 exhibited even more increased serum concentrations of urea, creatinine, sodium and potassium, blood pressure and microalbuminuria, and decreased creatinine clearance (p<0.05) in comparison with control group and unilateral nephrectomy.

CONCLUSION:

Progressive nephrectomy in rats seems to be useful to study the physiopathology of chronic kidney disease and its mechanisms of progression.

Key words:
Renal Insufficiency; Chronic; Nephrectomy; Models; Animal; Rats

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com

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[1] Correspondence: Ana Cristina Simões e Silva Laboratório Interdisciplinar de Investigação Médica Faculdade de Medicina, UFMG Avenida Alfredo Balena, 190/281 30130-100 Belo Horizonte - MG Brasil Tel.: (55 31)3409-8073 Fax: (55 31)3409-9770 acssilva@hotmail.com ana@medicina.ufmg.br

Renal function parameters (at two weeks)
Parameters	Control	Group 1	Group 2	Group 3
Serum Na⁺ (mEq/l)	141.3±2.3	144.0±2.6	155.8± 2.0^a	167.3± 2.8^a
Serum K⁺ (mEq/l)	4.3±0.23	4.7±0.5	5.7±0.3^a	6.8±0.3^a,b
Urine Na⁺ (mEq/l)	286.8± 28.2	240.3± 34.3	182.3± 25.2^a	142.3± 22.3^a,b
Urine K⁺ (mEq/l)	212.4± 32.3	194.0±26.3	153.9± 21.3^a	115.2± 18.3^a,b
Serum urea (mEq/l)	33.4±3.5	68.3±3.8^a	175.3± 8.8^a,b	230.4± 10.3^a,b
Serum creatinine (mEq/l)	1.1±0.2	1.8±0.4^a	5.7±1.2^a,b	7.9±1.1^a,b
Creatinine clearance (mL/min)	0.42±0.04	0.3±0.03^a	0.15± 0.01^a,b	0.07± 0.01^a,b
Microalbuminurina (mg/day)	0.42±0.04	10.3±0.8^a	48.7±3.7^a,b	78.6± 4.5^a,b

Renal function parameters (at four weeks)
Parameters	Control	Group 1	Group 2	Group 3
Serum Na⁺ (mEq/l)	142.3±2.1	146.0±2.8	160.8±2.3^a	172.3±2.6^a,b
Serum K⁺ (mEq/l)	4.1±0.23	4.6±0.5	5.9±0.4^a	6.9±0.38^a,b
Urine Na⁺ (mEq/l)	298.8±25.2	230.3±32.3	172.3±22.2^a	135.3±20.3^a,b
Urine K⁺ (mEq/l)	222.4±32.3	191.0±28.3	143.9±20.3^a	108.2±17.1^a,b
Serum urea (mEq/l)	30.2±3.5	65.3±3.9^a	185.3±8.5^a,b	205.4±24.3^a,b
Serum creatinine (mEq/l)	0.9±0.2	1.7±0.48^a	6.7±1.1^a,b	8.9±1.4^a,b
Creatinine clearance (mL/min)	0.46±0.08	0.3±0.05^a	0.1±0.03^a,b	0.06±0.02^a,b
Microalbuminurina (mg/day)	5.0±0.2	12.3±0.7^a	55.7±3.5^a,b	85.6±4.8^a,b

Mean arterial pressure (mmHg)
	Two weeks			Four weeks
Groups	Week 1	Week 2	Week 1	Week 2	Week 3	Week 4
Control	105 ± 7.2	108 ± 5.6	107 ± 8.3	106 ± 6.7	108 ± 5.2	107 ± 5.2
Group 1	110 ± 7.5	112 ± 7.3	112 ± 6.7	114 ± 8.2	112 ± 7.8	116 ± 7.5
Group 2	155 ± 4.3^a,b	160 ± 5.8^a,b	157 ± 6.3^a,b	160± 4.1^a,b	158± 6.8^a,b	161± 8.2^a,b
Group 3	163 ± 5.3^a,b	165 ± 5.8^a,b	160 ± 6.3^a,b	165± 6.3^a,b	162± 6.2^a,b	165± 6.7^a,b

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Characterization of an experimental model of progressive renal disease in rats1 1 Research performed at School of Medicine of Itajubá-MG, Brazil.

ABSTRACT

PURPOSE:

METHODS:

RESULTS:

CONCLUSION:

Characterization of an experimental model of progressive renal disease in rats^¹ 1 Research performed at School of Medicine of Itajubá-MG, Brazil.