Carcinogenesis in rats subjected to a new model ureterosigmoidostomy and treated with L-lysine

Santos, Alessandra Marques dos; Coelho, Joao Paulo Ferreira; Juanes, Camila de Carvalho; Azevedo, Rafael Barbosa de; Melo, Nayanna de Oliveira Ramos; Jamacaru, Francisco Vagnaldo Fechine; Dornelas, Conceição Aparecida

doi:10.1590/S0102-865020160120000003

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Acta Cirúrgica Brasileira

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ORIGINAL ARTICLES • Acta Cir. Bras. 31 (12) • Dec 2016 • https://doi.org/10.1590/S0102-865020160120000003 copy

Carcinogenesis in rats subjected to a new model ureterosigmoidostomy and treated with L-lysine^¹ 1 Research performed at Laboratory of Experimental, Department of Pathology and UNIFAC - Faculty of Medicine, Universidade Federal do Ceará (UFC), Brazil. Part of Master degree thesis, Postgraduate Program in Pathology. Tutor: Profa. Dra. Conceição Aparecida Dornelas.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

PURPOSE:

To evaluate the effects of L-lysine on the intestinal and urothelial epithelium of rats subjected to ureterosigmoidostomy (new model for surgical carcinogenesis).

METHODS:

Forty-two rats, 9 weeks of age, were divided into 6 groups. Animals in groups A, B, C were subjected to ureterosigmoidostomy (US) and treated with L-lysine, celecoxib and H₂O, respectively. Groups D, E and F (non-operated controls) received L-lysine, celecoxib and H₂O, respectively. The L-lysine dose was 150 mg/kg and that of celecoxib was 20 mg/kg. The colon was analyzed for the presence of aberrant crypt foci (ACF) under a stereomicroscope.The tissue was stained with hematoxylin and eosin and PAS alcian blue.

RESULTS:

There were rare ACF, and there was no statistically significant difference between the groups. Histopathologic study of the ureteral epithelium identified moderate to severe urothelial hyperplasia in rats with ureterosigmoidostomy. Transitional hyperplasia in the ureters of animals receiving L-lysine (A) showed an apparent difference compared to the control (C) (P=0.2424). There was no dysplasia or atypia

CONCLUSION:

L-lysine does not promote carcinogenesis of the intestinal and urethelial epithelium of rats subjected to ureterosigmoidostomy at the doses and times studied.

Key words:
Urinary Diversion; Carcinogenesis; Lysine; Rats

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[1] Correspondence: Profa. Dra. Conceição Aparecida Dornelas Faculdade de Medicina, Departamento de Patologia e Medicina Legal Rua Monsenhor Furtado, s/n 60441-750 Fortaleza - CE Brasil Tel.: (55 85)3366-8300/3366-8301 eusoucondor@yahoo.com.br

Histopathologic findings	Group A		Group B		Group C		Significance (Chi-square)
Histopathologic findings	n	%	n	%	n	%
Inflammation
Absent	1	16.67	1	16.67	0	0.00	P = 0.5698
Present	5	83.33	5	83.33	6	100.00
Total	6	100.00	6	100.00	6	100.00
Degree of inflammation
Absent	1	16.67	1	16.67	0	0.00	P = 0.1644
Mild	0	0.00	1	16.67	3	50.00
Moderate	0	0.00	1	16.67	2	33.33
Severe	5	83.33	3	50.00	1	16.67
Total	6	100.00	6	100.00	6	100.00
Papilloma
Absent	4	66.67	6	100.00	5	83.33	P = 0.3012
Present	2	33.33	0	0.00	1	16.67
Total	6	100.00	6	100.00	6	100.00
Polyp
Absent	6	100.00	5	83.33	6	100.00	P = 0.3469
Present	0	0.00	1	16.67	0	0.00
Total	6	100.00	6	100.00	6	100.00
Degree of ureteral hyperplasia
Mild	0	0.00	1	16.67	0	0.00	P = 0.1797
Moderate	1	16.67	1	16.67	4	66.67
Severe	5	83.33	4	66.67	2	33.33
Total	6	100.00	6	100.00	6	100.00

Degree of ureteral hyperplasia	Group A		Group C		Total		Significance (Fisher's exact test)
Degree of ureteral hyperplasia	n	%	n	%	n	%
Mild / moderate	1	16.67	4	66.67	5	41.67
Severe	5	83.33	2	33.33	7	58.33	P = 0.2424
Total	6	100.00	6	100.00	12	100.00

Group	Aberrant crypt foci			Total
Group	Present	Absent
A	4	3	7
B	3	3	6
C	3	3	6
D	1	5	6
E	0	5	5
F	0	6	6
Total	11	25	36