Wound healing of diabetic rats treated with <i>Moringa oleifera</i> extract

Wistar rats were used (6 rats/group). Group 1 received normal saline (NS) v.o. Group 2 received moringa extract (100mg/kg v.o) for 3 weeks. Groups 3 and 4: Streptozotocin (STZ) induced diabetes. Group 3 received NS; Group 4 received aqueous ME (100mg/kg) v.o.The wounds of groups 1 and 3 rats were topically treated with NS; wounds of groups 2 and 4 treated with 200µL of 10% ME. After anesthesia, all rats had skin square excision wounds 1.5cm². Wound percent contractions were measured. On 10th day, blood glucose and serum cytokines were measured. Histometry of wounds was studied using ImagePro6.0 software.

Results:

Glycemia was significantly reduced in ME treated rats. These rats had higher percent contraction of the wounds on 2^nd, 5^th and 10^th days, then controls (p<0.05). Diabetic rats treated with NS had TNF-α, IL-1β and IL-6 expression higher than in rats receiving ME. The histopathological score of ME treated diabetic rats (198±13.7) was significantly higher than treatment with NS (145±10.5).

Conclusion:

ME extract positively influenced healing of wounds in diabetic rats after systemic and topical treatment.

Key words:
Wound Healing; Diabetes Complications; Moringa oleifera; Rats

	2^nd DAY	10^th DAY
	Glycemia (mg/dL)	Glycemia (mg/dL)
C/NS	145.1±12.3^a	152.3±10.8 ^b
C/ME	158.4±11.8 ^a	123.6±10.4 ^a
DIAB/NS	287.6±18.4 ^b	268.3±16.7 ^a
DIAB/ME	302.5±20.2 ^a	207.9±14.2^a

Groups	2^nd Day	5^th Day	10^th Day
C/NS	1%^a,,b	15%^a,b	61%^a
C/ME	15%^a	32%^a	92%
DIAB/NS	0,8%	25%	64%^b
DIAB/ME	12%^b	28%^b	88%^a,b

Groups	TNF α (pg/ml)	IL-1β (pg/ml)	IL-6 (pg/ml)
C/NS	274.5±26^a	77.2±16^a	140.5±23^a
C/ME	136.4±18^a	43.3±11^a	108.2±12^a
DIAB/NS	312.3±21^a	98.3±14	83.6±13
DIAB/ME	215.7±24^a	67.3±12^a	64.2±9^a

Groups	Wound scores
C/NS	186±8.3^a
C/ME	274±11.8^a,c
DIAB/NS	145±10.5^a,b
DIAB/ME	198±13.7^b,c

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com

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[1] Correspondence: Aldo Cunha Medeiros Avenida Nilo Peçanha, 620 59012-300 Natal - RN Brasil cirurgex.ufrn@gmail.com