Revista de Odontologia da Universidade de São Paulo
Print version ISSN 0103-0663
SOUZA, Ana Paula de; TREVILATTO, Paula Cristina; ZAIA, Alexandre Augusto and LINE, Sérgio Roberto Peres. Analysis of the matrix metalloproteases in duct-ligated submandibular salivary glands of rats. Rev Odontol Univ São Paulo [online]. 1999, vol.13, n.2, pp. 135-139. ISSN 0103-0663. http://dx.doi.org/10.1590/S0103-06631999000200006.
The purpose of the present study was to analyze the expression of the major metalloproteases in normal and ligated glands. The major metalloproteases detected had a molecular weight ranging from 72 to 65 kDa. The gelatinolytic activity increased progressively reaching a peak between 5 and 10 days after ligation. Bands with molecular weight ranging from 92-80 kDa were also detected in the capsule of the gland. The 92-80 kDa enzymes were only detected 2 days after ligation. The activity of these enzymes was supressed by specific inhibitors. Our results show that matrix metalloproteases play an active role in the extensive remodeling of the connective tissue that occurs during the atrophy of the submandibular gland.
Keywords : Metalloproteases; Salivary glands; Atrophy.