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Journal of the Brazilian Chemical Society

versión On-line ISSN 1678-4790


FREIRE, Renato S.; PESSOA, Christiana A.; MELLO, Lucilene D.  y  KUBOTA, Lauro T.. Direct electron transfer: an approach for electrochemical biosensors with higher selectivity and sensitivity. J. Braz. Chem. Soc. [online]. 2003, vol.14, n.2, pp. 230-243. ISSN 1678-4790.

The most promising approach for the development of electrochemical biosensors is to establish a direct electrical communication between the biomolecules and the electrode surface. This review focuses on advances, directions and strategies in the development of third generation electrochemical biosensors. Subjects covered include a brief description of the fundamentals of the electron transfer phenomenon and amperometric biosensor development (different types and new oriented enzyme immobilization techniques). Special attention is given to different redox enzymes and proteins capable of electrocatalyzing reactions via direct electron transfer. The analytical applications and future trends for third generation biosensors are also presented and discussed.

Palabras clave : direct electron transfer; electrochemical biosensors; electrodes; redox enzymes; self-assembled monolayer.

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