Journal of the Brazilian Chemical Society
On-line version ISSN 1678-4790
FERREIRA, Edilene C.; SOUZA, Gilberto B. and NOGUEIRA, Ana Rita A.. FIA-FAAS method for tannin determination based on a precipitation reaction with hemoglobin. J. Braz. Chem. Soc. [online]. 2003, vol.14, n.2, pp. 329-333. ISSN 1678-4790. http://dx.doi.org/10.1590/S0103-50532003000200023.
A flow system, coupled with flame atomic absorption spectrometry (FIA-FAAS), was developed for tannin determination in pigeon pea samples, exploring the precipitation reaction between tannins and proteins. Sample extracts obtained by sonication with a 50% (v/v) methanol solution were introduced into the system and induced to react with a hemoglobin solution. The precipitate produced was retained on a filter located in the analytical flow. A reversed flow of 1% (w/v) sodium dodecyl sulfate solution was used for solubilization of the precipitate from the filter and to conduct the tannin-hemoglobin complex to the FAAS, to quantify the iron ions present in the hemoglobin structure. A tannic acid solution was used to prepare the analytical curve. The proposed method allowed determination of 30 samples per hour, a standard deviation of 9.7% (n=10), and a quantification limit of 0.27 mg L-1 for tannic acid.
Keywords : tannin-protein complexes; hemoglobin; FIA; flame atomic absorption spectrometry.