Self-immobilized and radiation-immobilized stationary phases were prepared with poly(methyloctylsiloxane) on eight silica supports having different particle sizes, particle shapes and pore sizes. Columns prepared by the two immobilization procedures had similar efficiencies, resolutions and separation factors but columns with self-immobilized stationary phases had lower percent loadings and, thus, lower retention factors. Results show structural differences between the phases.
silica; radiation immobilization; stationary phase; poly(metyloctylsiloxane)
