Journal of the Brazilian Chemical Society
On-line version ISSN 1678-4790
BONATO, Pierina S. and PAIAS, Fernanda O.. Enantioselective analysis of omeprazole in pharmaceutical formulations by chiral high-performance liquid chromatography and capillary electrophoresis. J. Braz. Chem. Soc. [online]. 2004, vol.15, n.2, pp. 318-323. ISSN 1678-4790. http://dx.doi.org/10.1590/S0103-50532004000200025.
We developed two sensitive and simple assay procedures based on high- performance liquid chromatography and capillary electrophoresis (CE) for the enantioselective analysis of omeprazole in pharmaceutical formulations. Rac-omeprazole and (S)-omeprazole were extracted from commercially available tablets using methanol:NaOH 2.5 mol L-1 (90:10, v/v). Chiral HPLC separation of omeprazole was obtained on a CHIRALPAK AD column using hexane:ethanol (40:60, v/v) as the mobile phase and detection at 302 nm. The resolution of omeprazole enantiomers by CE was carried out using 3% sulfated b-cyclodextrin in 20 mmol L-1 phosphate buffer, pH 4.0 and detection at 202 nm.
Keywords : omeprazole; enantiomer; chiral HPLC; chiral CE; pharmaceutical formulation.