SciELO - Scientific Electronic Library Online

 
vol.15 issue2Determination of cadmium by flame-atomic absorption spectrometry after preconcentration on silica gel modified with cupferronHot-filament metal oxide deposition (HFMOD): a novel method for depositing thin films of metallic oxides author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Journal of the Brazilian Chemical Society

Print version ISSN 0103-5053

Abstract

BONATO, Pierina S.  and  PAIAS, Fernanda O.. Enantioselective analysis of omeprazole in pharmaceutical formulations by chiral high-performance liquid chromatography and capillary electrophoresis. J. Braz. Chem. Soc. [online]. 2004, vol.15, n.2, pp. 318-323. ISSN 0103-5053.  http://dx.doi.org/10.1590/S0103-50532004000200025.

We developed two sensitive and simple assay procedures based on high- performance liquid chromatography and capillary electrophoresis (CE) for the enantioselective analysis of omeprazole in pharmaceutical formulations. Rac-omeprazole and (S)-omeprazole were extracted from commercially available tablets using methanol:NaOH 2.5 mol L-1 (90:10, v/v). Chiral HPLC separation of omeprazole was obtained on a CHIRALPAK AD column using hexane:ethanol (40:60, v/v) as the mobile phase and detection at 302 nm. The resolution of omeprazole enantiomers by CE was carried out using 3% sulfated b-cyclodextrin in 20 mmol L-1 phosphate buffer, pH 4.0 and detection at 202 nm.

Keywords : omeprazole; enantiomer; chiral HPLC; chiral CE; pharmaceutical formulation.

        · abstract in Portuguese     · text in English     · pdf in English