The ultrasound assisted metal extraction with water soluble tertiary amines (CFA-C reagent) in presence of hydrogen peroxide was used for improving metal solubilization in fish samples. Two species of freshwater fish (Pseudoplatystoma coruscans e Salminus brasiliensis), differing basically in lipid content were used. The optimization step was carried out using a factorial experimental design. The proposed procedure allowed the quantitative extraction of Ca, Cu, Fe, Mg, and Zn. In the validation process accuracy was evaluated by comparison with the total acid digestion of the sample in Parr a digestor and using a certified reference material (Fish homogenate, MA-A-2, IAEA). All results were in agreement at a 95% confidence level according to a paired-t test.
extraction; fish muscle tissue; metal; ultrasound; tertiary amines
