SciELO - Scientific Electronic Library Online

 
vol.16 issue6AThallium trinitrate-mediated ring contraction of 1,2-dihydronaphthalenes: the effect of electron-donating and electron-withdrawing groupsPlatinum complexes with non-symmetric fluorodithioethers: Molecular structures of cis-[PtCl2(CH3SCH2CH 2SRf)] (Rf = C6F5, C6H4F-3) and trans-[PtCl2(CH3SCH2CH 2SC6F5)2] author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Journal of the Brazilian Chemical Society

Print version ISSN 0103-5053

Abstract

SILVA JR., Luiz Severo et al. Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection. J. Braz. Chem. Soc. [online]. 2005, vol.16, n.6a, pp. 1174-1178. ISSN 0103-5053.  http://dx.doi.org/10.1590/S0103-50532005000700013.

An easy and simple chromatographic method for the determination of riboflavin in bovine milk in the presence of tetracyclines was established. For this, the proteins were precipitated with trichloroacetic acid and the separation was carried out by reversed-phase HPLC with fluorescence detection. A C8 column was used with isocratic flow using a mobile phase consisting of 0.1 mol L-1 sodium acetate, 35 mmol L-1calcium chloride and 25 mmol L-1 EDTA : methanol (65:35 v/v). The method was validated by means of the following parameters: linearity (0.9980), linear range (0.50 - 1.30 µg mL-1), intra- and inter-assay precision (RSD < 0.5%), accuracy (recovery 92%), and detection and quantitation limits (0.75 µg mL-1 and 0.22 µg mL-1, respectively). The method was applied for analysis of full cream and skimmed milk, showing robustness in the presence of tetracyclines, and when compared with a spectrofluorimetric method showed no significant difference (p < 0.05) between the results obtained.

Keywords : riboflavin; milk; tetracyclines.

        · abstract in Portuguese     · text in English     · pdf in English