Journal of the Brazilian Chemical Society
versión impresa ISSN 0103-5053
PEREIRA, Elisabete A.; MICKE, Gustavo A. y TAVARES, Marina F. M.. Development and validation of a capillary electrophoresis method for the determination of sulfate in indinavir sulfate raw material. J. Braz. Chem. Soc. [online]. 2006, vol.17, n.2, pp. 251-256. ISSN 0103-5053. http://dx.doi.org/10.1590/S0103-50532006000200006.
In this work, a simple and fast capillary electrophoresis method using indirect UV detection (230 nm) for the quantitative determination of sulfate in the indinavir sulfate raw material was described. A running electrolyte consisting of 10 mmol L-1 ammonium molybdate containing 0.15 mmol L-1 cetyltrimethylammonium bromide (pH 7.5) was used. Samples were injected hydrodynamically by applying 5000 Pa pressure during 3 s. The response was linear from 10.1 µg mL-1 to 79.8 mg µL-1. Limits of detection and quantification were 0.34 and 1.13 mg µL-1, respectively. The intra-day precision expressed as relative standard deviation was 2.8% for 10 independent sample preparations. Recoveries varying from 96.9 to 102.4% at three concentration levels were obtained. The methodology was successfully applied for real samples. The concentration of sulfate in indinavir sulfate raw material varied from 13.3 to 13.5% (m/m calculated as anhydrous base).
Palabras llave : indinavir sulfate; capillary electrophoresis; sulfate; validation; HIV.