Journal of the Brazilian Chemical Society
versión impresa ISSN 0103-5053
HOFFMANN, Andrea A. et al. Cationic dyes immobilized on cellulose acetate surface modified with titanium dioxide: factorial design and an application as sensor for NADH. J. Braz. Chem. Soc. [online]. 2007, vol.18, n.8, pp. 1462-1472. ISSN 0103-5053. http://dx.doi.org/10.1590/S0103-50532007000800003.
The electrochemical properties of meldola blue and toluidine blue cationic dyes immobilized on cellulose acetate surface modified with titanium dioxide were investigated by cyclic voltammetry. The materials synthesized were employed as carbon paste electrodes. The redox mediator properties of the meldola blue and toluidine blue chemically modified electrodes were optimized using a factorial design, consisting of two levels and four factors with two pseudo-central points (n= 20 experiments). The factorial analysis was carried out by searching for better reversibility of the redox process, such as the lowest separation between anodic and cathodic potential peaks and a current ratio near unity. The factors that presented significant effects on the overall optimization of the system to achieve the best conditions of the reversibility of electron transfer were the main factors scan rate and type of electrode (meldola blue or toluidine blue), besides the interaction factors KCl concentration × type of electrode (B×D) and the pH × [KCl] concentration × scan rate (A×B×C) interaction. The best electrochemical reversibility conditions obtained were: using the CA-TiO2-MB electrode, 1.0 mol L-1 KCl as supporting electrolyte, at scan rate of 10.0 mV s-1. Afterwards, the CA-TiO2-MB modified electrode was tested as an amperometric sensor for the determination of NADH, with a detection limit of 0.1 µmol L-1.
Palabras llave : meldola blue; toluidine blue; modified cellulose acetate; factorial design; carbon paste electrode; NaDH.