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Solid phase microextraction (SPME) analysis of volatile compounds produced by in vitro shoots of Lantana camara L. under the influence of auxins and cytokinins

The effects of the auxin indole-3-acetic acid at 0.44 µmol L-1 and the cytokinins thidiazuron at 0.44 µmol L-1 and benzyladenine at 0.44 and 4.4 µmol L-1 on growth, development and volatile compounds production of Lantana camara L. in vitro plants were investigated. The volatiles were extracted by solid phase microextraction (SPME) and the quantification was performed using GC-MS. A higher content of myrcene, alpha-phellandrene, alpha-copaene, trans-caryophyllene and beta-gurjunene, and the lower levels of alpha-pinene and beta-pinene were observed in in vitro plants cultivated on different supplementation media than in donor plants.

Lantana camara L.; volatile compounds; SPME; growth regulators; tissue culture


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