Journal of the Brazilian Chemical Society
On-line version ISSN 1678-4790
HAJIAN, Reza; SHAMS, Nafiseh and RAD, Ardeshir. Application of h-point standard addition method for simultaneous spectrophotometric determination of hydrochlorothiazide and propranolol. J. Braz. Chem. Soc. [online]. 2009, vol.20, n.5, pp. 860-865. ISSN 1678-4790. http://dx.doi.org/10.1590/S0103-50532009000500009.
The applicability of H-point standard addition method (HPSAM) to resolving overlapping spectra corresponding to the hydrochlorothiazide and propranolol was verified. The results show that the HPSAM is suitable for the simultaneous determination of hydrochlorothiazide and propranolol in aqueous media. The results of applying the HPSAM showed that the two drugs could be determined simultaneously with the concentration ratios hydrochlorothiazide:propranolol varying from 1:20 to 10:1 in a mixed sample. The proposed method has been successfully applied to the simultaneous determination of hydrochlorothiazide in the presence of propranolol in some synthetic samples. Moreover, the applicability of the method was demonstrated by their determination in some biological fluids.
Keywords : hydrochlorothiazide; propranolol; spectrophotometry; H-point standard addition method; HPSAM.