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Journal of the Brazilian Chemical Society

Print version ISSN 0103-5053On-line version ISSN 1678-4790


ŞANLI, Senem; ŞANLI, Nurullah  and  ALSANCAK, Güleren. Determination of protonation constants of some tetracycline antibiotics by potentiometry and lc methods in water and acetonitrile-water binary mixtures. J. Braz. Chem. Soc. [online]. 2009, vol.20, n.5, pp.939-946. ISSN 0103-5053.

An accurate estimation of dissociation constants of tetracycline antibiotics in acetonitrile-water binary mixtures is very important for several separation techniques such as liquid chromatography and capillary electrophoresis that use these solvent mixtures. In this study, the pKa values of five tetracyclines (tetracycline, oxytetracycline, chlortetracycline, doxycycline and metacycline), have been determined in water and acetonitrile-water binary mixtures (10%, 20% and 30% (v/v)) by potentiometry and liquid chromatography (LC). The obtained results show a similar trend for the pKa1 values of five tetracycline antibiotics as they increase with increasing concentration of organic modifier, which allows a linear relationship between pKa1 values and mole fraction of acetonitrile to be obtained. The pKa values obtained in aqueous medium have been compared with the values predicted by the SPARC on-line pKa calculator.

Keywords : tetracyclines; dissociation constants; potentiometric titration; LC.

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