Resumo

HAJIAN, Reza; SHAMS, Nafiseh  e  MOHAGHEGHIAN, Majid. Study on the interaction between doxorubicin and Deoxyribonucleic acid with the use of methylene blue as a probe. J. Braz. Chem. Soc. [online]. 2009, vol.20, n.8, pp. 1399-1405. ISSN 0103-5053.  http://dx.doi.org/10.1590/S0103-50532009000800003.

In this work, the interaction of doxorubicin with calf thymus double strand Deoxyribonucleic acid (ds-DNA) has been investigated with the use of Methylene Blue (MB) dye as a probe by the application of UV-Vis spectrophotometry, voltammetry and spectrofluorometry. The voltammetric behavior of doxorubicin has been investigated at glassy carbon electrode using differential pulse voltammetry. Doxorubicin is reduced, yielding one reduction peak. Both UV-Vis spectrophotometry and differential pulse voltammetry studies confirm the intercalation reaction. The results showed that both doxorubicin and the MB molecule could intercalate into the double helix of the DNA. The apparent binding constant of doxorubicin with DNA has been found to be 3.2 × 10⁴ L mol^-1. The fluorescence signal of doxorubicin and methylene blue was quenched with DNA addition. The Stern-Volmer equation was plotted based on quenching fluorescence signal of doxorubicin.

Palavras-chave : doxorubicin; DNA; chemotherapy; spectrophotometry; voltammetry; spectrofluorometry.