Abstract

CHEENPRACHA, Sarot et al. Three new amides from Streptomyces sp. H7372. J. Braz. Chem. Soc. [online]. 2011, vol.22, n.2, pp. 223-229. ISSN 0103-5053.  http://dx.doi.org/10.1590/S0103-50532011000200006.

Three new amides, methyl phenatate A (1), actiphenamide (2) and actiphenol 1-β-D-glucopyranoside (3), along with thirteen known compounds, were isolated from the organic extract of a fermentation culture of Streptomyces sp. H7372. The structures were elucidated by spectroscopic methods including 1D- and 2D-NMR techniques, and MS analyses. Cycloheximide (6) and cyclo(ΔAla-L-Val) (8) gave a clear zone of inhibition of Ras-Raf-1 interaction in the yeast two-hybrid assay which showed high potency with 10 and 25 µm clear ZOIs on SD His^- and inactive on SD His⁺ at 2.5 µg per disk, respectively.

Keywords : Streptomyces sp; yeast two-hybrid screen; Ras-Raf-1 interaction; amides; Cyclo(ΔAla-L-Val).