SciELO - Scientific Electronic Library Online

vol.22 número9Effect of temperature on methylene blue decolorization in aqueous medium in electrical discharge plasma reactorGalvanostatic removal of Pb2+ ions from diluted solutions by the use of a membrane-less flow-through cell with stainless steel wool electrodes índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Journal of the Brazilian Chemical Society

versión impresa ISSN 0103-5053


BEIGI, Farzaneh Ahmad Khan; IMANI, Mohammad; PAYEHGHADR, Mahmood  y  HOSSEINI, Hedayat. SPE-HPLC method for determination of ketoconazole and clotrimazole residues in cow's milk. J. Braz. Chem. Soc. [online]. 2011, vol.22, n.9, pp.1679-1685. ISSN 0103-5053.

A simple confirmatory method for HPLC-UV determination of ketoconazole and clotrimazole residues in cow's milk after solid phase extraction (SPE) is reported in this article. The samples were deprived of proteins and lipids by treating with acetonitrile and consequent extraction in n-hexane. Extracts were further cleaned-up and concentrated via solid phase extraction. The analytes were determined quantitatively using a validated high performance liquid chromatography method. The method was linear in the range of 0.1-1.0 µg mL-1 for both analytes. Limits of detection and quantification for both analytes were equal to 0.01 and 0.1 µg mL-1, respectively. Important parameters influencing the extraction efficiency were investigated and then optimized; the proposed method was applied to the analysis of milk samples. Satisfactory recoveries were obtained in the range of 95.9-101.78% using SPE.

Palabras clave : ketoconazole; clotrimazole; solid-phase extraction; high performance liquid chromatography; milk.

        · resumen en Portugués     · texto en Inglés     · Inglés ( pdf epdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons